Journal: Pathogens

Article Title: Methodologies for Generating Brain Organoids to Model Viral Pathogenesis in the CNS

doi: 10.3390/pathogens10111510

Figure Lengend Snippet: Commonly used cell types for CNS organoid generation. * price is for academic or non-profit institutions.

Article Snippet: KYOU-DXR0109B Human IPS Cells [ ] , USD 1623 , Cerebral , ATCC.

Techniques: Generated

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Circ_0043265 is downmodulated in TSCC tissues and cell lines. (A) Expression of circ_0043265 in TSCC tissues and their adjacent non-tumor tissues. (B) The survival curve of TSCC patients with low and high circ_0043265 expression based on median circ_0043265 level in TSCC tissues. (C) Relative expression levels of circ_0043265 in HOK and TSCC cell lines. The expression levels of circ_0043265 were upmodulated in SCC25 (D) and Cal27 (E) cells transfected with Lv-circ_0043265, as determined using qRT-PCR. ** P < 0.01 vs. control (adjacent non-tumor tissues, HOK, Lv-control).

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Control

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Effects of circ_0043265 overexpression on the cell proliferation, migration and invasion of TSCC cells. Effects of circ_0043265 overexpression on the cell proliferation of SCC25 (A) and Cal27 (B) cells, as determined using CCK-8. Effects of circ_0043265 overexpression on cell invasion of SCC25 (C) and Cal27 (D) cells, as determined via a Matrigel-coated Transwell assay. Effects of circ_0043265 overexpression on the cell migration of SCC25 (E) and Cal27 (F) cells, as determined via a Transwell assay. Representative pictures of the cell migration in SCC25 (G) cells were manifested. * P < 0.05, ** P < 0.01 vs. Lv-control.

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Over Expression, Migration, CCK-8 Assay, Transwell Assay, Control

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Circ_0043265 expression is inversely correlated with miR-1243 expression in TSCC. A predicted binding site of miR-1243 within circ_0043265 using Circular RNA Interactome. WT and MUT targeting regions of circ_0043265 for miR-1243 (A) . Effects of circ_0043265 overexpression on the expression of miRNAs that possessed putative binding sites for circ_0043265 in SCC25 (B) and Cal27 (C) cells. Expression levels of miR-1243 in TSCC and their adjacent non-tumor tissues (D) . Association between miR-1243 and circ_0043265 in TSCC tissues (E) . Expression levels of miR-1243 in TSCC cells and HOK (F) . ** P < 0.01 vs. control (Lv-control, adjacent non-tumor tissues, HOK).

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Expressing, Binding Assay, Over Expression, Control

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Circ_0043265 directly downmodulates miR-1243 expression in TSCC cells. Effects of circ_0043265 on the expression of miR-1243 in SCC25 (A) and Cal27 (B) cells. Relative luciferase activity in SCC25 (C) and Cal27 (D) cells co-transfected with circ_0043265-WT or circ_0043265-MUT luciferase plasmid and control mimic or miR-1243 mimic. Detection of circ_0043265 in RNA pulled down via Bio-circ_0043265, Bio-circ_0043265-MUT or Bio-NC probes. Detection of miR-1243 in RNA pulled down via Bio-circ_0043265, Bio-circ_0043265-MUT or Bio-NC probes in SCC25 (E) and Cal27 (F) cells. ** P < 0.01 vs. control (Lv-control, control mimic, Bio-NC-probe).

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Expressing, Luciferase, Activity Assay, Transfection, Plasmid Preparation, Control

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Upmodulation of miR-1243 reverses the effects of circ_0043265 on the cell proliferation, invasion and migration of TSCC cells. Expression of miR-1243 in SCC25 (A) and Cal27 (B) cells transfected with Lv-control + control mimic, Lv-circ_0043265 + control mimic or Lv-circ_0043265 + miR-1243 mimics, as determined via qRT-PCR. Proliferation of SCC25 (C) and Cal27 (D) cells transfected with Lv-control + control mimic, Lv-circ_0043265 + control mimic or Lv-circ_0043265 + miR-1243 mimics, as determined via a CCK-8 assay. The cell invasion ability of SCC25 (E) and Cal27 (F) cells transfected with Lv-control + control mimic, Lv-circ_0043265 + control mimic or Lv-circ_0043265 + miR-1243 mimics, as determined via a Matrigel-coated Transwell assay. The cell migration ability of SCC25 (G) and Cal27 (H) cells transfected with Lv-control + control mimic, Lv-circ_0043265 + control mimic or Lv-circ_0043265 + miR-1243 mimics, determined via a Transwell assay. ** P < 0.01 vs. Lv-control + control mimic.

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Migration, Expressing, Transfection, Control, Quantitative RT-PCR, CCK-8 Assay, Transwell Assay

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Circ_0043265 facilitates SALL1 expression via sponging miR-1243. A predicted binding site of miR-1243 within the SALL1 3′-UTR region using Targetscan (A) . Luciferase reporter assays were used to evaluate the interaction between SALL1 3′-UTR and miR-1243 in SCC25 (B) and Cal27 (C) cells. Relative mRNA levels of SALL1 in miR-1243-overexpressed SCC25 (D) and Cal27 (E) cells were measured using qRT-PCR. Relative protein levels of SALL1 in miR-1243-overexpressed SCC25 and Cal27 cells were measured using western blots (F) . Relative mRNA levels of SALL1 in circ_0043265-overexpressed SCC25 (G) and Cal27 (H) cells were measured using qRT-PCR. Relative protein levels of SALL1 in circ_0043265-overexpressed SCC25 and Cal27 cells were measured using western blots (I) . Expression levels of SALL1 mRNA in TSCC and the adjacent non-tumor tissues (J) . Spearman correlation analysis between SALL1 and circ_0043265 expressions in TSCC tissues (K) . Spearman correlation analysis between SALL1 and miR-1243 expressions in TSCC tissues (L) . ** P < 0.01 vs. control (Control mimic, Lv-control, adjacent non-tumor tissues).

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Expressing, Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot, Control

Journal: Pathology and Oncology Research

Article Title: Hsa_circ_0043265 Restrains Cell Proliferation, Migration and Invasion of Tongue Squamous Cell Carcinoma via Targeting the miR-1243/SALL1 Axis

doi: 10.3389/pore.2021.587130

Figure Lengend Snippet: Knockdown SALL1 reverses the inhibitory effects of circ_0043265 on the cell proliferation, migration and invasion of TSCC cells. SALL1 mRNA and protein expression in SCC25 (A) and Cal27 (B) cells transfected with Lv-control + control mimic, Lv-circ_0043265 + control mimic or Lv-circ_0043265 + miR-1243 mimics. Cell proliferation of SCC25 (C) and Cal27 (D) cells transfected with Lv-control + siRNA NC (si-NC), Lv-circ_0043265 + si-NC or Lv-circ_0043265 + SALL1 siRNA, as determined via a CCK-8 assay. The cell invasion ability of SCC25 (E) and Cal27 (F) cells transfected with Lv-control + si-NC, Lv-circ_0043265 + si-NC, or Lv-circ_0043265 + SALL1 siRNA, as determined via a Matrigel-coated Transwell assay. The cell migration ability of SCC25 (G) and Cal27 (H) cells transfected with Lv-control + si-NC, Lv-circ_0043265 + si-NC, or Lv-circ_0043265 + SALL1 siRNA, as determined via a Transwell assay. ** P < 0.01 vs. Lv-control + control mimic or si-NC.

Article Snippet: Human TSCC cell lines (SCC25, SCC15, SCC9 and Cal27) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and human oral keratinocytes (HOK) were prepared from outgrowth cultures of mucosal biopsies with appropriate ethical approval.

Techniques: Knockdown, Migration, Expressing, Transfection, Control, CCK-8 Assay, Transwell Assay